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Copyright: UCT Prague 2017
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Deepening of cooperation in bioactive compounds research (BIOCOM)

The proposed project aims at deepening of cooperation in common research activities between two universities in Czech Republic and Norway, University of Chemistry and Technology, Prague (UCT Pragure) and UiT The Arctic University of Norway (UiT), respectively. Strengthening and improvement of activities in the field of biologically active compounds research is planned. The partners will benefit from sharing and transfer of complementary scientific competences. UiT team is highly recognized for its credit in the field of bioprospecting, i.e. discovery and commercialization of ´new´ products based on biological resources, specifically marine. For this purpose, advanced biotechnologies, including isolation and purification of bioactive compounds are being developed by UiT jointly with industrial partners. On the other hand, UCT Prague is known for the excellence in the field of advanced, comprehensive analysis of natural matrices, what is the field which UiT is going to develop to reduce the need of outsourcing analytical services. The experience in employing cutting edge instrumental platforms for both bioactive compounds identification and quality / safety / stability parameters control will be the UCT´s project input. Another area for close collaboration and generic knowledge transfer is bioactivity testing. UiT employs an array of biochemical and cellular tests that might be implemented at UTC that has recently bought a robotic platform for this purpose. Both young scientists (PhD students), and experienced academics and senior research staff will increase their research profiles and enhance the skills in interdisciplinary communication through series of short-term trainings / study stays, expert’s visits and participations in events organised by project partners. The cooperation will also enable establishing the new collaborative research teams which will follow the present research, and will also support future extension of the collaboration to include research projects focusing on exploitation of natural resources in general.

 

 

The project "Deepening of cooperation in bioactive compounds research" (BIOCOM)  benefits from a 791 000 CZK grant from Norway Grants. The aim of the project is to stimulate long-term cooperation, networking, knowledge sharing and transfer, capacity and competence building in the research field focused on biologically active compounds.

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FACTS about the project:

   
Project title:
Deepening of cooperation in bioactive compounds research
 
Project coordinator:
Prof. Jana Hajslova,  Ph.D., UCT Prague
 
Total budget: 791 000 CZK
 
Project duration:            
1 February 2017 – 30 April 2017
 
Project No: 7F16028
 
CZ09 - Czech-Norwegian Research Programme
 

NORWAY GRANTS 

Through the Norway Grants and EEA Grants, Norway contributes to reducing social and economic disparities and to strengthening bilateral relations with the beneficiary countries in Europe. Norway cooperates closely with the EU through the Agreement on the European Economic Area (EEA).
For the period 2009-14, Norway’s contribution is €1.7 billion. Grants are available for NGOs, research and academic institutions, and the public and private sectors in the 12 newest EU member states, Greece, Portugal and Spain. There is broad cooperation with Norwegian entities, and activities may be implemented until 2016.
Key areas of support are environmental protection and climate change, research and scholarships, civil society, health and children, gender equality, justice and cultural heritage.
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Description of initiatives

 

Initiative 1

Activity 1

2 short-term trainings / study stays of PhD students from UCT Prague at UiT to gain the knowledge about (a) the marine resources as animals, bacteria, microalgae, plants etc. for use in bioprospecting, (b) suitability and application of different methods for the use in research related to bioprospecting, (c) bioavailability, bioactivity and potential toxicity assessment; (d) biotechnologies producing final product based on bioactive compounds

(i) Practical training in the UiT laboratory to get familiar with the strategies employed in bioprospecting research including sample handling, description, storage, prior- analysis processing, bioactivity and potential toxicity testing;  up-scaling of laboratory practices into real-life biotechnology scale. More information here.

(ii) Participation in PhD training course (PhD Course BIO-8009: Marine bioprospecting and bioactive compounds for health) organised by UiT. More information here.

 

Activity 2

Short-term training / study stay of PhD students from UiT at UCT Prague

Practical training in the lab to gain knowledge on advanced analytical strategies based on employing ultra-performance liquid chromatography coupled to tandem high resolution mass spectrometry (UPLC-HRMS/MS) for identification of biologically active compounds in biological matrices. Optimization of analytical method, using various databases for compounds identification, chemometric data processing employing multi variate statistics

 

Activity 3

Experts´ visit of UCT Prague research staff at UiT

Academic staff from UCT Prague attending the Norwegian UiT will gain new experience leading to strengthening of bilateral relations; (i) will explore new opportunities for research collaboration, particularly possibilities for common project proposals in the field of food and biotechnology sciences, (ii) will plan common research activities leading to preparation of scientific paper(s). More information here.

 

Activity 4

UiT representative’s participation and presentation at the conference organised by UCT Prague

Prof. Stensvag will participate with keynote lecture on topic related to the project activities in the conference Chemical Reactions in Foods VIII (CRF 2017, www.crf2017.eu) organised by UCT Prague on 15-17 February 2016 in Prague. More information here.

 

Initiative 2

Organisation of a workshop on topic related to biologically active compounds in natural resources. More information here.

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The Arctic University of Norway, Tromso (UiT)

The Faculty of Biosciences, Fisheries and Economics

   

Key people at NMBU

Klara Stensvåg, Professor

Klara-Stensv-g-2008-006 (originál)Dr. Klara Stensvåg is professor in Marine Biotechnology form The UiT The Arctic University of Norway, Tromsø. She took her PhD in 1998. Her main research focus is use of different marine bioresources and she has been working on fish immunology and defense molecules in marine invertebrates. The main activity in ongoing projects is marine bioprospecting and drug discovery with a particularly focus of antimicrobial compounds and bioactive peptides. She has been a member of a national scientific board in biotechnology and teach in Marine Biotechnology.

 

 

 

 

 

University of Chemistry and Technology, Prague (UCT)

Department of Food Analysis and Nutriton

 

Key people at UCT

Jana Hajslova, Professor

Photo JH_small (originál)

Prof Jana Hajslova is the head of the Department of Food Analysis and Nutrition, University of CHemistry and Technology, Prague (UCT Prague) and chairs Accredited (ISO17025) Laboratory "Food Quality and Safety". She is a widely recognized expert in the field of food / environmental chemistry and analysis; she has published more than 250 original papers on development of advanced analytical strategies of contaminants, residues, natural toxins and other biologically active compounds, authentication and metabolomics. Her research team has participated in many international and national projects at both research and management levels, including the EC 5 - 7th Framework Programme, HORIZON 2020, several COSTs and EEA and Norwegian grants. Prof Hajslova together with her PhD students has been involved in various bilateral international research activities; under her supervision, close collaboration with many world-renowned institutions, such as WHO, FAO, USDA and the European Commission’s Joint Research Centre has been established. Currently she is the Czech Republic delegate in HORIZON 2020 SOCIETAL CHALLENGE 2: "Food Security, Sustainable Agriculture and Forestry, Marine and maritime and inland water research and the Bioeconomy" committee. As the chairwomen, she had a key input in establishing a series of very reputable international symposia "Recent Advances in Food Analysis" series in 2003-2015.

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UCT_basic (originál)

University of Chemistry and Technology, Prague (UCT Prague)
Department of Food Analysis and Nutrition
Technicka 3
Prague 6
16628
Czech Republic

Contact persons of the institution:

Martina Vlckova
e-mail: martina.vlckova@vscht.cz
Tel: +420220443180

 

 LOGO UiT (originál)

The Arctic University of Norway, Tromso (UiT)
The Faculty of Biosciences, Fisheries and Economics
P.O. Box 6050 Langnes
Tromso
9037
Norway

Contact persons of the institution:

prof. Klara Stensvag, Ph.D.
mail: klara.stensvag@uit.no
Tel: +4777644512

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Description of initiatives

 

Initiative 1

Activity 1

2 short-term trainings / study stays of PhD students from UCT Prague at UiT to gain the knowledge about (a) the marine resources as animals, bacteria, microalgae, plants etc. for use in bioprospecting, (b) suitability and application of different methods for the use in research related to bioprospecting, (c) bioavailability, bioactivity and potential toxicity assessment; (d) biotechnologies producing final product based on bioactive compounds

(i) Practical training in the UiT laboratory to get familiar with the strategies employed in bioprospecting research including sample handling, description, storage, prior- analysis processing, bioactivity and potential toxicity testing;  up-scaling of laboratory practices into real-life biotechnology scale. More information here.

(ii) Participation in PhD training course (PhD Course BIO-8009: Marine bioprospecting and bioactive compounds for health) organised by UiT. More information here.

 

Activity 2

Short-term training / study stay of PhD students from UiT at UCT Prague

Practical training in the lab to gain knowledge on advanced analytical strategies based on employing ultra-performance liquid chromatography coupled to tandem high resolution mass spectrometry (UPLC-HRMS/MS) for identification of biologically active compounds in biological matrices. Optimization of analytical method, using various databases for compounds identification, chemometric data processing employing multi variate statistics

 

Activity 3

Experts´ visit of UCT Prague research staff at UiT

Academic staff from UCT Prague attending the Norwegian UiT will gain new experience leading to strengthening of bilateral relations; (i) will explore new opportunities for research collaboration, particularly possibilities for common project proposals in the field of food and biotechnology sciences, (ii) will plan common research activities leading to preparation of scientific paper(s). More information here.

 

Activity 4

UiT representative’s participation and presentation at the conference organised by UCT Prague

Prof. Stensvag will participate with keynote lecture on topic related to the project activities in the conference Chemical Reactions in Foods VIII (CRF 2017, www.crf2017.eu) organised by UCT Prague on 15-17 February 2016 in Prague. More information here.

 

Initiative 2

Organisation of a workshop on topic related to biologically active compounds in natural resources. More information here.

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Keynote lecture in the conference CHEMICAL REACTIONS IN FOODS VIII (CRF 2017) within the topic Bioprospecting strategies, composition and safety characterization, authentication / fraud detection, traceability of origin, shelf-life testing

  

BIOACTIVE COMPOUNDS FROM MARINE SOURCES

Klara Stensvag, The UiT The Arctic University of Norway, Tromso, Norway

  

Marine fish is an important source for food in the world, but there is also unused marine resources that we know less about in terms of the biomass and basic knowledge of bioactive molecules that might be usable for different purposes. Many of the drugs approved today is based on knowledge from natural products (over 60%) from terrestrial sources. During the last decades, however, there has been a growing interest for bioprospecting of marine resources for their unique bioactive properties in addition for being usable as food or food products. Marine organisms like animals and bacteria together with other biological material of marine origin, are a source of huge potential for exploring novel bioactive components with activities that can be exploited. In this context, marine proteins and peptides are very interesting. The wide repertoire of biological functions that such natural peptides have, make them exciting for bioprospecting and drug discovery. Among them, antimicrobial peptides (AMPs) are interesting since the increased spreading of bacterial resistance in humans and other pathogenic bacteria worldwide against commercial available antibiotics, have stimulated the search for novel antimicrobials. We have characterized several new classes of marine AMPs and explored their mode of action. The organisms that produce these bioactives have been collected from the Arctic or/and sub-Arctic region and can be very diverse covering biological resources from microalgae to invertebrates. Different approaches and examples in bioprospecting will be looked into. This will include characterizations of mechanisms of action, SAR studies and designed new marine mimicking molecules that might be candidates for novel lead compounds.

Keywords: marine organisms, bioactive components, marine proteins and peptides, bioprospecting

Acknowledgement: The research leading to these results has received funding from the Norwegian Financial Mechanism 2009-2014 under Project Contract n° MSMT-743/2017-73-11.

 

CRF_Stensvag (originál)          CRF_Stensvag 2 (originál)

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Report of training within the project " Deepening of cooperation in bioactive compounds research" (BIOCOM)

 

Name of trainees:                                                          Prof. Ing. Jana Hajšlová, CSc., doc. Ing. Milena Stránská, PhD.

Home Institution:                                                          University of Chemistry and Technology, Prague (UCT Prague)
                                                                                               Faculty of Food and Biochemical Technology
                                                                                               Department of Food Analysis and Nutrition

Address of home institution:                                    Technická 3
                                                                                               Prague 6 Dejvice
                                                                                               166 28
                                                                                               Czech Republic

Name of supervisor in the home institution:     Prof. Ing. Jana Hajšlová, CSc.

Visited project partner:                                                UiT The Arctic University of Norway (UiT)

Address of project partner:                                         UiT Norges arktiske universitet
                                                                                                 Postboks 6050 Langnes
                                                                                                 9037 Tromsø

Name of supervisor in the project partner:          Prof. Klara Stensvåg

Actual period of visit:                                                     April 23 – April 26, 2017

 

Short summary of the training

The travel to Tromso realized within the project “Deepening of cooperation in bioactive compounds research” was undertaken between April 23rd and April 26th 2017.

On Monday (April 24th) prof. Jana Hajslova, Ph.D. and assoc. prof. Milena Stranska, Ph.D. (University of Chemistry and Technology in Prague) had a meeting with Marek Cuhra, Ph.D. (Institute of Marine Research, Tromso). Possibilities of future collaboration focused mainly on bioprospecting of marine organisms, and utilization of antimicrobial compounds in medical applications were discussed.

The next day (April 25th), prof. Jana Hajslova, Ph.D. and assoc. prof. Milena Stranska, Ph.D. were invited to UiT The Arctic University of Norway, where a common brainstorming seminar focused predominantly on identification of common scientific topics (manly within HORIZON 2020) was organized. The main Norwegian scientists participating on the seminar were prof. Klara Stenswag and prof. Ragnar Olsen (the complete list of participants is available). Additionally to this, also collaboration of both Universities at the educational level (e.g. within ERASMUS program), and creation of common Ph.D. study program, were outlined. The communications will go on continuously, between the respective responsible persons or university departments.

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WORKSHOP on "Deepening of cooperation in bioactive compounds research"

  

Description of the workshop

The workshop on a topic related to biologically active compounds in natural resources will be organised by UCT Prague in March 2017. The workshop will be “cross-connecting in science”, with the aim to create new research groups and establish the new topics for the follow up research. This will significantly contribute to the sustainability of Norwegian and Czech partner cooperation after the finishing of the project. The workshop will be attended by project participants (research staff, postdocs and PhD students) and also by external stakeholders who can be interested in the project actions and outputs. These organisations will be invited to extend collaboration of project partners with bodies also outside the project leading to strengthening links and networking that might be beneficial for cooperation in future projects. Program of the workshop will be planned with respect to invited audience and focus both on scientific aspects, and also presentation of opportunities for funding of research in the field of BIOCOM activities.

  

Programme of the workshop

 

Photo gallery

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Programme of the workshop

9:45 –10:00

Welcome coffee

10:00 – 10:10

Welcome, Jana Hajslova, Head of Department of Food Analysis and Nutrition, UCT Prague & Pavel Ulbrich, vice dean for research and development, Faculty of Food and Biochemical Technology, UCT Prague

Introduction of the workshop participants

10:10 – 10:20

Overview of BIOCOM activities

Jana Hajslova, project leader, UCT Prague

10:20 – 10:40

UCT Prague introduction

Jana Hajslova, UCT Prague

10:40 – 11:00

UiT introduction

Klara Stensvåg, UiT

11:00 – 12:00

Presentations on UCT Prague research activities related to the project topic

 

Kamila Hurkova (Metabolomics as an effective tool for assessment of food authenticity and novel food)

Marie Fenclova (Bioactive compounds studies: examples of research challenges)

Vojtech Hrbek (SFC-HRMS: a nove analytical technique for natural compounds, contaminants and metabolomics)

Monika Jiru (Microalgae as a source of organic selenium: new functional food production)

12:00 – 13:00

Presentation on UiT research activities related to the project topic

 

Klara Stensvåg

13:00 – 14:00

Lunch

14:00 – 15:00

INTERNAL PART OF THE WORKSHOP:

Discussion on project outcomes

Planning of common future activities

Moderators: Jana Hajslova, Klara Stensvåg

… The cooperation will also enable establishing the new collaborative research teams which will follow the present research, and will also support future extension of the collaboration to include research projects focusing on exploitation of natural resources in general.

15:00

End of the workshop

18:00

Networking dinner

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Report of training and workshop within the project "Deepening of cooperation in bioactive compounds research" (BIOCOM)

 

Name of trainees:                                                          Darina Lanková, Veronika Krtková, Monika Jírů, Marie Bícová

Home Institution:                                                           University of Chemistry and Technology (UCT) Prague

                                                                                                Faculty of Food and Biochemical Technology

                                                                                                Department of Food Analysis and Nutrition

Address of home institution:                                     Technická 3, Prague 6 Dejvice, 166 28, Czech Republic

Name of supervisor in the home institution:      Prof. Ing. Jana Hajšlová, CSc.

Visited project partner:                                                UiT The Arctic University of Norway (UiT)

Address of project partner:                                         UiT Norges arktiske universitet, Postboks 6050 Langnes, 9037 Tromsø

Name of supervisor in the project partner:         Prof. Klara Stensvåg

Actual period of visit:                                                     April 3.-9. 2017

 

Short summary of training:                                         

(In detail see attachment no. 1.)

Tuesday April 4                                                               

Tour around the laboratories at the Department of NFH, followed by the introduction to lab routines and tour in the laboratory facilities.   Short introduction to Marine bioprospecting and methods of bioassay guided purification. Demonstration of bioactivity testing of antimicrobial activity.

Wednesday April 5                                                        

The talk about searching for antimicrobial active components in marine organism. Lectures: „Pyridine and piperidine; Privileged heterocycles in drug discovery“ and „Synthetic mimics of antimicrobial peptides - Synthesis and biological studies of novel synthetic mimics of antimicrobial peptides“

Thursday April 6                                                             

Antimicrobial peptides and cases form own research. Mechanisms of actions studies. Introduction for preparation for biosensoric assays and mode of actions studies of antimicrobial compounds. Supply issues of natural products. Practical session doing two biosensoric assays of mechanisms of actions of antimicrobial activity.

Friday April 7                                                                  

Seminar with presentation of PhD projects.

Titles:                                                                              Organohalogen contaminants in human environment (Darina Lanková)

                                                                                           Biological active compounds of Cannabis plant (Veronika Krtková)

                                                                                           Opium alkaloids in capsules and seeds of Poppy (Papaver somniferum L.) (Marie Bícová)

                                                                                           Food fraud of botanical-based dietary supplements (Monika Jírů)

  

Foto 1 (originál)

  

Foto 2 (originál)

  

Foto 3 (originál)

  

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Foto 6 (originál)

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Report on transfer knowledge obtained during the training session into trainees’ institution

 

Course: Tromsø, 27. 2. - 10. 3. 2017

Trainee: Kamila Hůrková

Trainers: Professor K. Stensvåg (leader of the course), Professor M. Jaspars, Professor J. H. Andersen, Professor T. Brautaset, Assiciated professor T. Haug, Associated professor B. E. Haug, Dr. P. Nichols, Dr. Ch. Li, Dr. E. Mischenko, Postdoctor K. Ø. Hansen, Postdoctor T. Rämä, Researcher J. Isaksson

The course “ BIO-8604 Marine bioprospecting and bioactive compounds for health ”, administered by Faculty of Biosciences, Fisheries and Economics at UiT The Arctic University of Norway was a PhD student course held for 2 weeks and it was a mixture of preconference lectures (some online), a preconference workshop, practical sessions, and seminars with discussions. Part of this course was going on at the same time as the conference Bioprosp_2017. The conference and one preconference workshop were included in the course. The topics covered during the course:

  • Marine bioresources available for drug discovery and bioprospecting
  • The Marine Biodiscovery Pipeline
  • Overview of different methods used in drug discovery, spending from bioassay guided purifications, genetic approaches and virtual approaches
  • Different techniques and approaches;  how to purify components, solve structures and measure activity for instance in high throughput screening, peptidomics
  • Challenges and case studies of lead development
  • Legal aspects
  • Topics given at the conference and pre-conference workshop
  • Practical sessions with demonstrations

  

During the course I gained valuable and interesting insight into the bioprospecting pipeline from collection of the marine resources, preparation of sample extracts and extracts fractionation, screening of the fractions, isolation and identification of bioactive natural products, to biosynthesis of novel bioactive molecules. This knowledge will be implemented by our research group in our department for bioprospecting of terrestrial and fresh water algae and other material. Moreover, participation in this course will enhance prospects for my professional development in bioprospecting area. In addition, meeting with experts in the field and other people interested in bioprospecting provided an opportunity for networking.

Within this course I prepared a presentation on the topic related to the course “Fucoxanthin-producing algae in a bioprospecting context”. It was considered as the final exam of the course which I successfully passed (see the certificate).

I would like to thank everybody who participated in organising this course and the Bioprosp_2017 conference, mainly prof. Klara Stensvåg who ensured that my stay was full of scientific and personal experiences.

 

  Picture 1_Peter Nichols (originál)

Peter Nichols (CSIRO, Australia) presenting at the Scientific Workshop: Marine bioprospecting - Production of health promoting compounds and novel approaches in biodiscovery

Picture 2_networking (originál)

Networking: PhD students, participants of the Marine bioprospecting and bioactive compounds for health course discussing with Peter Nichols (CSIRO, Australia)

Picture 3_Keynote presentation (originál)

Keynote Presentation: Challenges and opportunities of bioprospection through microbial culture collections given by Jörg Overmann (Leibniz-Institut DSMZ, Germany)

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